Detection of coagulase negative staphylococii from goats with subclinical mastitis and determination of their antibiotic resistancy

In this study, a total of 100 sampling was made from 100 goat with subclinical mastitis in lactation period from Aydin Province region in summer time of year 2012, then samples were brought to Adnan Menderes University Faculty of Veterinary Medicine Department of Microbiology. A total of 67 (67 %) Coagulase Negative Staphylococci isolates were obtained out of 100 samples. In our study, 19 (28 %) of isolates were found as S. lentus, 17 (26 %) of isolates were found as S. warneri, 12 (18 %) of isolates were found as S. haemolyticus, 8 (12 %) of isolates were found as S. xylosus, 4 (6 %) of isolates were found as S. schliferi, 3 (4 %) of isolates were found as S. cohnii, 2 (3 %) of isolates were found as S. caprae and2 (3 %) of isolates were found as S. hyicus out of 67 isolates which were detected conventionally. In our study, 52 (78 %) of isolates were found resistant to Ampicillin, 17 (25 %) of isolates were found resistant to Cefaperazone, 55 (82 %) of isolates were found resistant to cloxacillin, 10 (15 %) of isolates were found resistant to danofloxacillin, 10 (15 %) of isolates were found resistant to enrofloxacillin out of 37 isolates. In addition, 1 (1.5 %) of the isolates were found intermediate susceptible to oxytetracyclin, 11 (16 %) of the isolates were found intermediate susceptible to enrofloxacillin and 8 (12 %) of the isolates were found intermediate susceptible to danofloxacillin. As the same samples were detected by polymerase chain reaction, out of 67 Coagulase Negative Staphylococci isolates, mecA gene was detected from 3 (4.5 %) of the isolates. mecA gene was detected from 2 (3 %) S. warneri isolates and from 1 (1.5 %) S. xylosus isolate.