Expression levels in various cell lines of naturalantisense transcripts

lncRNAs (long non-coding RNAs), with various important molecular and cellular functions and natural antisense transcripts (NATs), complementary to protein-coding or non-coding RNA sequences are important regulators of eukaryotic gene expression drew a great attention recent years to uncover their importance for diagnostic, prognostic and therapeutic purposes because of their dysfunctions leads to diseases including cancer.Earlier NATs were described as lncRNAs. This convergence between NAT and lncRNA determination raised confusion and gradually started to be disappeared with the increasing knowledge. Specific pcGen (protein coding Gene) regulation by their corresponding ncNATs (non-coding NATs) has been reported. TSIX is a new regulator of collagen expression which stabilizes the collagen mRNA.HOXD-AS1/ HAGLR is a critical regulator for carcinogenesis and metastasis in different types of cancers. LCMT-1 is a negative regulator of Akt proto-oncogene. Different tissue-specificity of NAV2AS5 wasobserved from human tissue samples. Antisense transcripts that bind to long noncoding RNAs are called lncNATs.Among the potential clinical utilities of lncNATs, are of high interest for cancer treatment, even though the biological significance remains still under scientific investigation with major key questions yet to be elucidated.We hypothesized that lncNATsTSIX, HAGLR, LMCT1AS, and NAV2AS5 might have important roles for several tumorigenic processes. To explore their roles, ATCC normal (Beas2B, CRL4010, CRL8798) and cancer (A549, MCF7, MDA-MB- 231, CRL2329) cell lines were subjected to examine through RNA isolation, cDNA conversion, Semiquantitative (by agarose gel and ImageJ program) and quantitative RT- PCRfor expression analyses. TSIX, HAGLR, LMCT1AS and NAV2AS5 genes have differential expression pattern in both normal and cancer cell lines. The obtained results indicate their importance for biological processes in cancer. Anahtar Kelimeler: Cancer, HAGLR, LCMT1AS, NAV2AS5, Natural Antisense RNAs. E.mail ( aishanku@yahoo.com)