By antimüllerian hormone assesment of ovarian reserve and effect of selenium to ovarian damage reduction with ischemia-reperfusion injury in rats

Aim: İnvestigation of ovarian reserve effected by ovarian torsione-detorsione. Determinining the relationship between ovarian reserve and AMH. And evaluating biochemistrical and histological parameters to ovarian ischemia-reperfusion and effect of SeleniumMaterial and Methods: This study made in Ege University Medicine Faculty after receiving approval from ethical committee This study contains young adult, approximately in 200-250gr weight, 50-female Wistar Albino rats. There are five groups in research. Group1:Sham, Group2(3T/D), Group3(3T/D+Se), Grup4(24T/D), Grup5(24T/D+Se). Selenium was administered intraperitoneally to rats (to Group 3 and 5) twenty minutes before detorsion operation. Ovarian ischemia-reperfusion injury evaluated by using biochemical (MDA, SOD, GSH-Px) and immunohistochemical parameters (eNOS, iNOS, Caspase 3, Cytochrom c, Apaf-1, Fas-L, TUNEL). In addition the number of follicles, histologic grade, AMH used to determine ovarian reserve.Results: Ischemia-reperfusion groups compared with sham group, using biochemical (MDA, SOD, GSH-Px) and immunohistochemical parameters for apoptosis (eNOS, iNOS, Caspase 3, Cytochrom c, Apaf-1, Fas-L, TUNEL). Prolonged time of ischemia-reperfusion associated with increasing ovarian damage (p<0,01). The data are evaluated, the prevention of ovarian damage, the efficacy of selenium, statistically significant at the p <0.01 was. But there was not statistically significant about Fas-L (in Group 3), and SOD (in Group 3 and 5). AMH changes between the groups was evaluated. According to degree of ovarian damage, and to duration of ischemia-reperfusion, p> 0.05 was found.Conclusion: Biochemical and immunohistochemical analysis showed that, prolonged time of ischemia-reperfusion associated with increasing ovarian damage. And also Selenium treatment was effective in preventing ovarian damage. In addition the using AntiMüllerian hormone, were not significant in detecting of increased ovarian damage (p>0,05).Key words: AntiMüllerian Hormone, Ovarian Reserve, İschemia-Reperfusion