Detection of mycoplasma synoviae in the region of izmir province by molecular methods

In this study; 375 tracheal swabs from five different commercial layer and broiler poultry in İzmir were examined by vlhA gene based PCR. In our study; at the end of the culture; M. synoviae were identified at the rate of 12.8 % from whole samples. The percentage of M. synoviae as per layer (1st, 2nd, 3rd) is shown as follows: 32.2 %, 29.3 %, 2.7 %. M. synoviae has not been detected at none of two different broilers. On the other hand; in the studies that has been done with vlhA based PCR 12 positive samples were detected per 375 samples. The percentage of M. synoviae as per layer (1st, 2nd, 3rd) is shown as follows: 1.3 % (1 positive sample), 6.7 % (5 positive samples), 8 % (6 positive samples). The samples taken from broilers had paralel results between cultured and PCR which were both negative. In our study; M. synoviae was detected with PCR in layers. But at the samples taken from broilers M. synoviae couldn't been identified. The study showed that PCR methods is faster and more reliable than cultured.