Evaluation of lncrna (lincrna-EPS) expression level in anemia associated with chronic kidney disease

Aim: In this study, we aimed to evaluate the expression levels of lincRNA erythroid prosurvival (lincRNA-EPS), which is one of the long non-coding RNA family, in patients with chronic kidney disease (CKD) related renal anemia and compare it with a healthy control group. We also aimed to investigate whether the expression levels of lincRNA-EPS are associated with anemia parameters (such as hemoglobin, MCV, ferritin, vitamin B12, folic acid) and erythropoietin (EPO) doses. Material and Methods: The total of 59 people were included in our study, with 40 patients and 19 control groups. Demographic characteristics of the patients were recorded. Blood samples were taken for laboratory analysis. Complete blood count and biochemical parameters were studied from the blood samples taken. Analyzes of lincRNA expression levels were studied by RT-qPCR. Patients diagnosed with stage 3-5 CKD and healthy groups were grouped. The data were analyzed using the SPSS package program. Results: The LincRNA-EPS expression median and quartile values were respectively median value: 0.000041; 1st Quartile (Q1): 0.000017, 2nd Quartile (Q2): 0.000041, 3rd Quartile (Q3): 0.00038 in the healthy control group; median value: 0.5255; 1st Quartile (Q1): 0.0072, 2nd Quartile (Q2): 0.5255, 3rd Quartile (Q3): 0.00038 in the patient group. As a result of these data, lincRNA-EPS expression values were found to be higher in the group with CKD (p <0.001). In addition, negative correlation was found between the patients' EPO levels and lincRNA-EPS expression levels (r=-0.654, p=0.011). Conclusion: Our study is the first in the literature to evaluate the levels of lincRNA-EPS in CKD patients. Considering the increased lincRNA-EPS expression level and the inverse correlation between EPO use and lincRNA-EPS expression level in CKD patients with renal anemia; In order to facilitate erythropoiesis, the increase in lincRNA-EPS expression level and suppression of proapoptotic gene Pycard, seems to be a possible mechanism. Key words: Chronic kidney disease, renal anemia, erythropoietin, long non-coding RNA