Association of NKT cell numbers with vitamin D receptor polymorphism in patients with multiple myeloma

Multıpl myeloma (MM) are hematological neoplasms with proliferation of malignant plasma cells in the bone marrow (13).MM is a neoplastic cell disorder in approximately %10 of all hematological neoplasm sand %2 of all canser deaths.Role of pathological conditions in controlling NKT cells in tümor growth in vivo . As in the immunoregulatory role of vitamin D3 in theprobe. Mutations mayoccur as a result of a nucleotıde change from one nucleotide to another (single nucleotide change - SNP) , or as a result of the addition or addition of a nucleotide to the DNA sequence .Most of the biological effects of vitamin D are fraught with an intra cellular receptor (VDR).Among the seidentified polymorphisms, rs1544410(Bsml) is located in intron 8, rs731236 (TaqI) in exon 9.Nucleotide gene polymorphisms in there sept or gene . The purpose of our study is to determine the number of NKT cells, which is important in immünity against canser in MM, and there lationship between the number of NKT cell sand VDR polymorphism (TaqI, BSML) in MM. The study group consists of 30 patients , including twenty men and ten women. It was composed of ten patients, including women. BSML and TaqI gene polymorphisms were studdied by Real-time PCR on the vitamin D receptör gene by isolation of purified DNA from these individuals. Inaddition , in blood sample sontained fromall cases ,by flow cytometry method ,total lymphocytes , CD3+ (Total T lymphocyte ),CD3+/CD4+(T helper) ,CD3+/CD8+(T cytotoxics ), CD3-/CD56 +(NK) ,CD3+/CD56+(NKT cells) were detected. When the study and control groups were compared, there was a significant differnce in total T lymphocyte ,T help erand cytotoxic lymphocytes. In the study group ,the homozygous GG allele was found to be high erthan the control group with a rate of %53.3 and the TT allele with a rate of %63.3 in terms of BSML and Taq I polymorphism ,while AA %16.6 and CC %13.3 mutant alleles were lower than the control group. Event hough there sultsobtainedreveal a relationship between MM and VDR polymorphism ,furtherstu dies with larger caseseries are needed to talk about the existence of thisrelationship. KEYWORD:1-Multıple myeloma 2-Vitamin D reseptor polymorphism 3-BSML 4-TaqI 5-CD3