Investigation of biofilm and virulence affect on salmonella serotypes

In our research, biofilm and virulence genes affect on Salmonella serotypes is investigated. Biofilm related existence of bapA, sdiA, invA, agfD and adrA genes of Salmonella serotypes is investigated with PCR method. For research, 44 Salmonella isolates taken from different sources and consist of 10 Salmonella Typhirium, 10 Salmonella Enteritidis and 24 Salmonella İnfantis. 38 (86.36%) of investigated 44 serotypes contain bapA gene, 26 (59%) contain sdiA gene, 44 (100%) contain invA gene, 39 (88.64%) contain agfD gene and 35 (79.55%) contain adrA gene. Despite the consistency of detected virulence genes characteristics through phenotypic method and PCR technique, for some characteristics results were higher with PCR technique than phenotypic method. This states that multigene related characteristic investigations can benefit from molecular researches and using both methods together is essential to produce more reliable results. With this research, regarding that Salmonella is a pathogen for human kind and causes lethal epidemics around the world, the necessity of applying hygienic rules from raw material to final product and taking precautions to avoid cross contamination at food production areas is presented.