The investigation of the effect of propolis on nitrosative stress in the brai̇n of total head irradi̇ated rats

RATS Nour ALAFANDI Master Thesis, University of Gaziantep, Institute of Medical Sciences Department of Medical Biochemistry Thesis Supervisor: Prof. Dr. Seyithan TAYSI August 2017, 77 pages In this study, we aimed to investigate the protective effects of propolis on nitrosative stress resulting from ionizing radiation. Thirty-six male rats were used. The groups consisted of 4 subgroups: 2 control, radiotherapy (R), Propolis + R. All of the groups except the control groups received a single dose of 5 Gy on the first day of radiotherapy. Biochemical parameters of the brain tissue were measured by spectrophotometry to determine whether protective effects of propolis were present. As a result of the study, it was determined that NO, NOS and ONOO- values were significantly higher in the brain tissue from the rats in the radiotherapy group than in the Propolis + R group. These results suggest that the use of the substance against the harmful effects of ionizing radiation, which has a protective effect against the adverse effects of nitrosative stress caused by propolis radiation, will provide beneficial results. Keywords: Radiation, Propolis, Nitric oxide, Nitric oxide synthase, Peroxynitrite