Phenotypic and genotypic analysis of antibiotic resistance profiles of bacteria isolated from blood culture samples

Sepsis, which is common and often lethal, is a serious public health problem. There is consensus that the incidence is increased in patients with sepsis due to an aging population, increased use of immunosuppressive therapy, and high-risk interventions. 750 patients with suspected sepsis hospitalized in intensive care units in the Health Education University Van Training and Research Hospital were evaluated. 750 patients were classified according to their age and sex. Bacteria were isolated from their blood cultures. Biochemical tests such as catalase test, oxidase test and Gram staining were performed. Vitek 2 Compact (Biomerieux, USA) device was used for identification of bacteria and evaluation of the antibiogram test. Slime factor was positive in 47 of the coagulase negative bacteria. The mecA, blaOXA-48 and blaIMP genes were analyzed by polymerase chain reaction (PCR). Twelve multidrug-resistant bacteria were isolated and identified from patients with sepsis. 2 MRSA isolates were positive for mecA gene carriage. Of our gram negative bacteria, only 2 K. pneumoniae isolates were found to be blaOXA-48 carriers. No blaIMP gene was found in any of our gram negative bacteria causing sepsis. In conclusion, identification of the bacteria causing sepsis in our hospital and determining the antibiotic resistance rates were found quite important. Among the causative agents of sepsis, effect of the presence of methicillin, carbapenem, enlarged β-lactamase and multidrug resistance bacteria on human health were found to be very important.