Pterjiumda WASP geninin analizi

Analysis WASP Gene Expression in Pterygium. Tokat Gaziosmanpaşa University, Health Sciences Institute, Department of Medical Biology, MSc. Thesis, Tokat. Pterygium is a benign growth of the conjunctive tissue that covers the sclera that is proliferative and causes intrusive ocular surface and wide matrix remodeling. The WASP gene is located on the p arm (short arm) of the X chromosome at locations Xp11.4→ p11.21 and encodes a 502 amino acid sequence. The WASP gene comprises 12 exons, and the protein has a molecular weight of about 65 kD. The WASP gene has multiple distinct domains, including a GTPase, GBD, PH domain, cofilin, and verprolin homology domain, SH3 binding motifs with a proline-rich locus, and the C-terminal acidic region, which plays a role in actin cytoskeleton remodeling by beginning Arp2/3. The WASP gene appears to play a function in signal transduction as well as cytoskeletal remodeling. Actin restructuring, platelet formation/removal, cell-cell contact, actin polymerization, cell trafficking and motility, immunological synapse arrangement, and neutrophil formation/release are all linked to WASP. The goal of this research was to see if the WASP gene was expressed in pterygium.Tissues were removed throughout surgery with the patient's agreement and knowledge. By collecting cDNA from the tissue levels established by real-time PCR, we employ the WASP gene expression technique. WASP gene expression levels were determined to be statistically significant higher in tissues with pterygium compared to the control group (p < 0.005), based on our findings that the increase in WASP gene expression is hypothesized to be linked to the formation and development of pterygium. According to qRT-PCR data, WASP gene expression is pterygium tissue compared to healthy conjunctive tissue (1.9100.367-foldchange, p=0.0001).