The examination of protective effects of aniseon ischemia reperfusion injury of testiciles in rats

Aim: Testicular torsion is a clinically common pathological condition that can lead to permanent damage and requires urgent treatment and surgical intervention. To date, many experimental studies have been conducted to prevent or treat ischemia-reperfusion injury resulting from testicular torsion. In this study, we aimed to examine the biochemical, histopathological, and immunohistochemical effects of anise in the treatment of ischemia-reperfusion injury in rat testis tissue. Material and Methods: In this study, 48 male Wistar Albino rats were used. The animals were divided into 6 equal groups: Group 1 is the control group (n=8) and no procedure was applied during the experiment. Group-2; (n=8), rats were administered 5mL/kg/day peroral (p.o.) anise by gavage for 30 days. Group 3: Rats testices were rotated 270o and testis ischemia was applied for 30 minutes. Then, reperfusion was supplied by derotation of the testicles. Group 4: Following 30 minutes of testicular ischemia and 30 minutes of reperfusion, rats were administered 5mL/kg/day peroral (p.o.) anise by gavage for 30 days. Grup 5: Rats were administered 5mL/kg/day peroral (p.o.) anise by gavage for 30 days, then 30 minutes of testicular ischemia and 30 minutes of reperfusion was applied tor at testicles. Grup 6: Rats were administered 5mL/kg/day peroral (p.o.) anise by gavage for 30 days, then 30 minutes of testicular ischemia and 30 minutes of reperfusion was applied tor at testicles, then rats were administered 5mL/kg/day peroral (p.o.) anise by gavage for another 30 days. Results: There was no difference in the histopathological evaluation of the control and anise groups. In the ischemia-reperfusion group, the damage was found to be the highest. In the I/R+Anise group, it was observed that the breakage and separation of the basement membrane continued in some places, while there was an increase in the number of spermatogenic cells compared to the I/R group. In the anise+I/R group, edema and congestion persisted. Anise+I/R+Aniseed group, on the other hand, was observed to be very close to the Control and Anise groups. Conclusion: We found that anise has beneficial effects such as anti-apoptotic and anti-inflammatory in the treatment of ischemia-related damage in rat testicles.