Correlation of intracytoplasmic sperm injection results with the proliferation and apoptosis index of cumulus cells

Today, the pregnancy rate of the patients who used assisted reproductive technics (IVF, ICSI) is under the influence of numerous factors. Some of the factors are quality of semen, type of the ovarian stimulation, grade of estradiol (E2) and age of the patients. These ones effect quantity and quality of the oocyte, rate of maturation and embryo development. Cumulus cells are a kind of strong regulator for ovarian cells in proliferation and apoptosis, effect oocyte by the local productions, manage maturation of oocyteIn this study, we have determined rates of proliferation and apoptosis for cumulus cells, according to age of the patients that seperated to two groups as ? 37 and ? 38 and evaluated correlation among results of the microenjectionDue to the aim, cumulus cells that were taken from cumulus-oocyte complex in the patients who are applied microenjection, proliferation index was determined by proliferating cell nuclear antigen (PCNA) monoclonal antibody. According to morphologic criteria under the microscope rate of the apoptosis was determined after the 4, 6 diaminido 2 phenilindol (DAPI) fluoresan stainingAccording to the findings, rates of proliferation of the cumulus cells were so high meaningfully for first group as ? 37. When rates of apoptosis of the cumulus cells have evaluated, difference between two groups is not significant. Furthermore maturation of oocyte, fertilization and pregnancy rate have evaluated on the groups that were determined rates of the proliferation and apoptosis. It was determined that total oocyte number and maturation rates as well as pregnancy rates increased significantly for the patients who have high proliferation index on the cumulus cells.In our study, we come to the conclusion that the proliferation rates of cumulus cells are a potentially indicator, will be applied for election of quality oocyte, which is a significant parameter in order to become healthy pregnant on the patients that are applied microenjection.