Investigation of the interaction between HSA-MİR-664A-5P and lncrna MEP3 in osteosarcoma

Osteosarcoma is the most common primary bone tumor in children and adolescents. Understanding the basic molecular mechanisms in developing cancer can be helpful in developing alternative treatment strategies. The relationship between dysregulated non-coding RNAs' (ncRNA) expression level and osteosarcoma was detected. Among those ncRNAs, the expression levels of hsa-miR-664a-5p were detected to be upregulated and MEG3 long non-coding RNA levels were detected to be downregulated in osteosarcoma tissue and cell lines. In this study, hsa-miR-664a-5p inhibitor was used in order to investigate the changes in the levels of MEG3 gene and hsa-miR-664a-5p in osteosarcoma cancer cell line (U2-OS) and human osteoblast cell line (hFOB 1.19). According to our results, the expression level of MEG3 gene was increased while the expression level of hsa-miR-664a-5p was decreased, as expected. The results are found to be statistically significant (p<0.05). As a result of this study, it was shown that the upregulated expression of hsa-miR-664a-5p could have a inhibitory effect on MEG3 gene expression in osteosarcoma. Key words: hsa-miR-664a-5p, lncRNA, MEG3, miRNA, Osteosarcoma