Histological evaluation of the effect of mecsina hemostopper on bone regeneration in critical size defects in rats

Reconstruction of the defects in the oral and maxillofacial region to reprovide aesthetic, phonation and function to the patients, is one of the most challenging procedures in oral and maxillofacial surgery. The aim of this study is to evaluate the recovery of hard tissue healing after application bovine xenogenic bone graft and Mecsina Hemostopper using as a herbal blood stopper. For this purpose; critical size defects were created on the calvarial bone region of 28 Sprague Dawley male rats. The groups are Mecsina group with only Mecsina Hemostopper, Mecsina + Graft group with Mecsina and Graft, Graft group with only Graft and control group without any agent were used. Critical size defects were filled with 0.1 cc liquid Mecsina and 0.12 cc Graft. On the 28th day following surgery, all animals underwent sacrification and the samples were evaluated histopathologically and histomorphometrically. As a result of histopathological evaluation, more bone marrow and bone formation and more vascular proliferation were observed in the groups where Mecsina was used; histomorphometric evaluation showed that trabecular and osteoid thickness was higher in Mecsina groups and it was found to provide higher quality bone formation statistically. (p <0,05) In this study, Mecsina was found to be an effective agent in increasing bone healing when applied alone or in addition to the graft. This study was aooroved by Baskent University Institutional Review Board (Project no: DA18/05) and supported by Baskent University Research Fund. Keywords: Critical Size Defect, Mecsina Hemostopper, Bone Healing, Xenogenic Bone Graft, Histomorphometric Analysis