Identification of the staphylococcus species which cause cattle mastitis with maldi-tof MS

The aim of this study was identification and antibiotic susceptibility of Staphylococcus species from mastitic milk, comparison of results by using commercial identification methods such as API, VITEK, MALDI-TOF MS, PCR and confirmed Staphylococcal agents with sequence analysis. A total of 100 milk samples taken in sterile conditions were investigated for the presence of Staphylococcus, which were found to have clinical mastitis problems in animals in the milk province of Aydın province and surrounding areas. Identification of Staphylococcus species was performed by VITEK 2, VITEK (MALDI-TOF) MS, PCR and sequencing in addition to biochemical methods. Staphylococcus sp. was isolated from 34 % of the milk samples. Seven (20.60 %) of the isolates were Staphylococcus aureus, 10 (29.40 %) were Staphylococcus simulans, 8 (23.50%) were Staphylococcus epidermidis and 4 (11.80 %) Staphylococcus saprophyticus, 2 (5.90 %) were Staphylococcus chromogenes, 2 (5.90 %) were Staphylococcus hyicus and 1 (2.95 %) were Staphylococcus arlettae. As a result of the antibiogram test, 34 Staphylococcus sp. isolates were sensitive to Cefoxitin, Ciprofloxacin, Vancomycin, Tigecycline and Phosphomycin in the ratio of 100 % and resistant to Linezolide, Clindamycin and Tetracycline in the ratio of 85.2%, 100 % and 82.4% respectively. As a result; the reliability of the MALDI-TOF MS proteomic biomass spectrophotometry method has been demonstrated to be verifiable and feasible.