The effect of sodium deoxycholate materials on apoptotic, cytotoxic and DNA damage of breast cancer cell line

The Effect of Sodium Deoxycholate Materials on Apoptotic, Cytotoxic and DNA Damage of Breast Cancer Cell Line. University of Van Yuzuncu Yil, Institute of Health Sciences, Department of Medical Biology, M.Sc. Thesis, Van, 2019. Breast cancer is the most common cancer in women and the leading cause of cancer death worldwide. Chemotherapy is a treatment usually performed with antineoplastic drugs to reduce metastasis and shrink tumor. The aim of this study was to investigate the effect of sodium deoxycholate on the molecular mechanisms of cytotoxic, apoptotic and DNA damage in the breast cancer cell line (MCF-7). In this study, different doses of sodium deoxycholate are used in MCF-7 breast cancer cell line based on the hypothesis that it has the potential to affect cancer cells. Cytotoxic effect of sodium deoxycholate on MCF-7 breast cancer cells and healthy normal breast cells (CRL- 4010) was examined by MTT method for anticancer property detection. After determining the cytotoxic impact, the efficacy of active sodium deoxycholate dose on apoptosis and DNA damage was examined by apoptotic DNA ladder and Western Blot methods. In addition, oxidative stress index and cell migration tests were performed. As a result, it was determined that sodium deoxycholate showed cytotoxic effect on MCF-7 and CRL-4010 cells but did not damage the DNA of the cells, and the presence of several proApoptotic proteins causing apoptosis were determined. Sodium deoxycholate was found to significantly reduce the activity of paraoxonase and arylesterase enzyme in MCF-7 cancer cells, which can be considered as a predictive risk factor for cancer. Sodium deoxycholate was also found to delay cell migration over time. The results showed that Sodium deoxycholate has an antimetastatic effect in breast cancer cells and is a potential agent for further cancer studies.