Sperm tyrosine kinase activity analyzes with immunocytochemistry

Acrosome reaction and fertilization depends on the capacitation ability gained by the sperms while in the female reproductive tract. Sperms that gain capacitation ability and show hyperactivity can realize acrosome reaction and fertilization. It is reported that phospho tyrosine kinase activation is effective in achieving capacitation, acrosome reaction and hence in fertilization process and reactive oxygen species have an effect in achieving tyrosine kinase activation during this process.This study was conducted in two groups by selecting cases of normospermia and cases of leukocytospermia with increased levels of leukocytes due to reactive oxygen species in semen. Additionally both groups have been separated into two subgroups each, in order to evaluate the effects of reactive oxygen species that appear in the centrifuge operation used during sperm preparation process. Semen samples that were separated into two were prepared by using swim up method where centrifuge process was not used and concentration method where centrifuge process was used and sperm smears were prepared for immunocytochemistry. The prepared samples were marked with anti-phospho tyrosine monoclonal antibody by using streptavidin-biotin peroxidase method.In the evaluation process, it was observed that sperm tyrosine kinase activation was localized in the tail section of the sperms, and expression levels of each case showed individual variances. Among the normospermia cases, it was observed that the activation increased in the concentration group in relation to the swim-up group, but this increase was not significant. In the leukocytospermia cases also the concentration group had increased activation. No significant differences were observed between normospermia and leukocytospermia groups. Even though it can be said that both among the normospermia cases and leukocytospermia cases, reactive oxygen derivatives are effective in sperm tyrosine kinase activity, further studies should be held in order to explain individual differences and results.