The effects of local PRP and ozone application on tendon healing in rabbits repaired of achilles tendon

In this study, the effects of PRP and ozone therapy on tendon healing in the treatment of Achilles tendon rupture were evaluated. Three groups were formed with 8 rabbits in each group: Group 1 Repair (R), Group 2 Repair + PRP (RP), Group 3 Repair + Ozone (RO). An acute tear model was created by sharp dissection into the Achilles tendon. Then, tendon repair was performed using the modified Kessler technique. O2O3 gas mixture was injected into the Achilles tendon for a total of 4 weeks in the RP group and PRP in the RP group. The study was terminated in the 6th postoperative week. These experimental groups were included in histological and biomechanical study. In histological analysis, it was observed that the improvement was at the highest level in the ozone treatment, and the improvement scores of PRP treatment significantly increased compared to the control group. Tensile test was applied in the biomechanical analysis. In the statistical analysis, it was seen that the RO (repair + ozone) group had a statistically significant increase compared to the R (repair) group. (p<0.05) No statistically significant difference was found between the data in the three groups in the maximum elongation values. As a result, histological and biomechanical analyzes show that ozone therapy is effective on the healing of the Achilles tendon. It was seen that PRP treatment increased the improvement in histological evaluation, but did not statistically increase the strength compared to the control group in biomechanical analysis.