Determination the expression levels of mirna 204-5P, mirna 138-5P and mirna 133-3P in formaline fixed-paraffine embedded tissue samples of endometrial cancer and relationship with the prognosis

In our study, we aimed to elucidate the function of microRNAs which have been considered as prognostic biomarkers guiding for early diagnosis and individualized therapy of endometrial cancer (EC). In this context, we determined the expression levels of miRNA 204-5p, miRNA 138-5p and miRNA 133-3p in FFPE tissue samples of EC patients and compared the relative differences by considering prognostic factors. This study includes 49 patients with type 1 EC (1. patient group), 35 patients with type 2 EC (2.patient group) which had surgical staging with a diagnosis of EC and 45 patients undergone endometrial sampling for abnormal uterine bleeding (control group) in our clinic between January 2010–December 2014. Expression levels of selected miRNAs were evaluated by qRT-PCR. Then, the prognostic variables of disease were compared with the levels of their expression. Kolmogorov Smirnov, Mann Whitney U, Kruskal Wallis and Dunn tests were used for statistical analysis. A p value below 0,05 was accepted as significant. miRNA 204-5p expression levels were significantly increased in type 1 EC patients comparing to control and type 2 EC groups (p=values 0,017 and 0,001 respectively). miRNA 138-5p and 133-3p expression levels were significantly increased in type 1 and type 2 EC patients comparing to control group (p=values 0,001). But, there were no significant correlation between expression levels and prognostic variables of EC. This is the first study evaluating the levels of miRNA 138-5p expression in ECs. Our results suggest that these miRNAs might be oncomiRs in the molecular pathogenesis of EC.