Detectıon of Staphylococcus aureus ın the goats by bacterologıcal and molecular methods

In this study, a total of 400 sampling was made from 50 hair goat and 50 Saanen goat in lactation period from Aydin Province region in summer time of year 2010, then samples were brought to Adnan Menderes University Faculty of Veterinary Medicine Department of Microbiology. A total of 48 (12 %) Staphylococcus aureus isolates were obtained out of 400 samples.8 (16,0 %) of the milk-borne S. aureus isolates were obtained from Saanen goats, 19 (38 %) of the milk-borne S. aureus isolates were obtained from hair goats. 3 (6 %) of the skin-borne S. aureus isolates were obtained from Saanen goats, 4 (8 %) of the skin-borne S. aureus isolates were obtained from hair goats. 1 (2,0 %) of the nasal-borne S. aureus isolates were obtained from Saanen goats, 6 (12 %) of the nasal-borne S. aureus isolates were obtained from hair goats. 2 (4,0 %) of the vaginal-borne S. aureus isolates were obtained from Saanen goats, 5 (10,0 %) of the vaginal-borne S. aureus isolates were obtained from hair goats. Milk, nasal, skin, and vagina borne S. aureus isolates were detected higher from hair goats.As the same samples were detected by polymerase chain reaction, out of 48 isolates, sek staphylococcal enterotoxin gene was detected between 278 bp fragment from 13 (27,0 %) samples. Detected enterotoxin genes were obtained from milk isolates in the number of 10 (66,7 %), 2 (22) of the genes were obtained from nasal isolates, 1 (11,1 %) of the genes were obtained from skin isolates. 8 (42,1 %) of the genes were detected from milk-borne S. aureus isolates in hair goats, 2 (25,0 %) of the genes were detected from milk-borne S. aureus isolates in Saanen goats, 1 (5,2 %) of the genes were detected from skin-borne S. aureus isolates in hair goats, 2 (10,5 %) of the genes were detected from nasal-borne S. aureus isolates in hair goats.