Investigation of the relationship between sirt1 targeting miRNAs and sirt1 gene expression exposed to high levels of glucose in H9C2 cell line

The purpose of this study was to determine the role of SIRT1 gene expression and relationship between SIRT1 targeting miRNAs on hyperglycemia which characterizes diabetes. SIRT1 expression levels were analyzed by RT-PCR and Western Blot methods after the exposure of the H9c2 rat cardiomyocyte cells to high levels of glucose. Using miRNA target prediction algorithms, miRNAs that possibly target SIRT1 were identified. 86 miRNAs targeting SIRT1 gene identified after using detailed in silico analysis. miR-181d, miR-135a, miR-135b, miR-181b-1- 3p, miR-369-3p, miR-487b, miR-361, miR-204, miR-30d, miR-23a, miR-9 and miR- 323 expression levels elevated statistically significant. In our study it was found that SIRT1 protein levels were increased 1,9 fold in H9c2 exposure to normo glucose levels than higher glucose levels. We think that SIRT1 targeting miRNAs in development of diabetic cardiomyopathy and its prevention could be effective and our study sheds light to further comprehensive study.