The importance of mi̇cro-RNA i̇n pati̇ents wi̇th hepatocellular carci̇noma

Introduction and Aim: It is very important to determine hepatocellular carcinoma at an early stage for prognosis and patient management. There is still no ideal marker to detect hepatocellular carcinoma at early stage. MicroRNA-26, microRNA-192 and microRNA-122 may be a novel biomarker for hepatocellular carcinoma. Materials and methods: This study was performed in 2014-2016 at Gaziantep University, Faculty of Medicine, Sahinbey Hospital with new diagnosed 42 hepatocellular carcinoma and 45 control (Nonalcoholic fatty liver disease) patients. The peripheral blood was collected from patients and controls. MicroRNA-26 microRNA-192 and microRNA-122 levels were determined by using real-time PCR method. Results: Serume miR-26 amount in hepatocellular carcinoma group was significantly higher than the level found in the control group (p<0.0001). According to the area under the ROC curve (AUC:0,748, %95 CI p<0.0001) the patient group were identified for the cut-off value of miR-26. Serume miR-26 amount in hepatocellular carcinoma group was not significantly higher than the level found in the control group (p=0.181). MiRNA-192 could not be expressed by real-time-PCR. In addition, patient's other diagnostic, clinical and prognostic data were compared with miR-26 levels. Conclusion: In our study we showed amounts of circulating miR-26 in different clinical stages of hepatocellular carcinoma in patient's serume with real timePCR method, also we compared patient's circulating miR-26 with other diagnostic, prognostic and clinical values. MicroRNA's are thought to be novel serum biomarkers in cancer diagnosis, prognosis and follow-up. To become a novel biomarker, miR-26 needs large-scale studies. But serume miR-26 alone may not be a good biomarker to show diagnosis, prognosis and surveillance of hepatocellular carcinoma. To use other diagnostic, clinical, prognostic indicators in conjunction with miR- may be more meaningful. Key Words: Hepatocellular carcinoma, microRNA-26, microRNA-192,microRNA-122.