Investigation of the effect of denosumab and ozone application on bone healing in critical size bone defects

Aim: In maxillofacial surgery, various drugs are used in order to accelerate the recovery of defects caused by any reason. Several studies have shown that denosumab from bisphosphonate group drugs used in osteoporosis patients has positive effects on new bone formation. In general medicine and dentistry, ozone is in widespread use as an alternative treatment and has positive effects on new bone formation. The aim of this study was to evaluate new bone formation by performing denosumab and ozone on critical-sized rat calvarium defects. Material and Method: In our study, 40 Sprague Dawley rats were used. Rats were divided into 4 groups. Only grafts were placed in the control group. After applying graft to ozone group (O) and ozone and denosumab (O-D) groups, topical ozone was applied for 15 seconds. Denosumab group (D) and ozone and denosumab (O-D) group were injected subcutaneously (s.c) 10 mg / kg Prolia (denosumab) every 4 weeks for 8 weeks. 5 animals from each group at the end of week 4, while the other five animals in the group at the end of 8 weeks after being sacrificed for histopathological examination was performed. The differences between the groups were evaluated by statistical analysis. Results: After histopathological examination, better bone formation was observed in the ozone and denosumab treated groups compared to the control group. There was no statistically significant difference between the groups except for the control group, however, new bone formation was determined in the groups treated with denosumab compared to the ozone group. Conclusion: As a result of our study, we believe that the application of ozone and denosumab has a positive effect on the formation of new bone, but we think that more studies should be done. Key Words: rat, ozone, denosumab, new bone formation